›› 2012, Vol. 24 ›› Issue (5): 356-358.doi: 10.3969/j.issn.1004-616x.2012.05.008

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Detection of serum reproductive hormone in cynomolgus monkey embryo - fetal developmental toxicity evaluation model

YUAN Fang;LI Bi-hai;PAN Xiao-liang;LIN Hai-xia;WANG Qing-li;CHANG Yan   

  1. (1. National Shanghai Center for New Drug Safety Evaluation and Research，Shanghai 201203；2. South-China Primate Research and Development Center，Guangdong Entomological Institute，Guangzhou 510555；3. Center for Drug Evaluation，SFDA，Beijing 100038，China)
  • Received:2011-11-29 Revised:2012-05-01 Online:2012-09-30 Published:2012-09-30
  • Contact: CHANG Yan

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Abstract: OBJECTIVE: The study was performed to evaluate change of reproductive hormone level during early pregnancy in cynomolgus monkeys，and to collect background data to support the cynomolgus monkey embryo-fetal developmental toxicity evaluation model. METHODS：Blood samples from cynomolgus monkeys on gestation day 0(GD0) and gestation day 20(GD20) were collected to measure serum progesterone levels. Blood samples from pregnant monkeys were collected once a week during GD27-100 to measure serum estradiol，progesterone and prolactin levels by chemiluminescence method. RESULTS：Progesterone levels of pregnant female monkeys on GD20 were significantly higher than GD0. Progesterone level rose slowly from GD0 to GD27 until a peak，decreased to the trough on GD48，and then gradually rose until GD100. Estradiol level increased slowly from GD0 to GD27 until a peak，decreased to the lowest level on GD34，and then gradually rose until GD100. Prolactin level began to rise slowly from GD0 to GD69，and remained stable. CONCLUSION：Reproductive hormone levels in cynomolgus monkeys changed dynamically during early pregnancy. Progesterone levels can be used as supportive evidenced of early pregnancy.

Key words: cynomolgus monkey, progesterone, estradiol, prolactin